Orthotopic Model of Hepatocellular Carcinoma in Mice - Centre de recherche des Cordeliers
Chapitre D'ouvrage Année : 2024

Orthotopic Model of Hepatocellular Carcinoma in Mice

Résumé

Orthotopic models of hepatocellular carcinoma (HCC) consist in the implantation of tumor cells into the liver by direct intrahepatic injection. In this model, tumorigenesis is triggered within the hepatic microenvironment, thus mimicking the metastatic behavior of HCC. Herein, we detail a surgically mediated methodology that allows the reproducible and effective induction of liver-sessile tumors in mice. We enumerate the steps to be followed before and after the surgical procedure, including HCC cell preparation, the quantity of cancer cells to be injected, presurgical preparation of the mice, and finally, postoperative care. The surgical procedure involves laparotomy to expose the liver, injection of cells into the left-lateral hepatic lobe, and closure of the incision with sutures followed by wound clips. We also provide information concerning the subsequent tumor growth follow-up, as well as the application of bioluminescence imaging to monitor tumor development.
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Dates et versions

hal-04596615 , version 1 (05-09-2024)

Identifiants

Citer

Flavia Lambertucci, Sijing Li, Omar Motiño, Léa Montégut, Uxía Nogueira-Recalde, et al.. Orthotopic Model of Hepatocellular Carcinoma in Mice. Liver Carcinogenesis, 2769, Springer US, pp.1-13, 2024, Methods in Molecular Biology, ⟨10.1007/978-1-0716-3694-7_1⟩. ⟨hal-04596615⟩
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