Biodegradation by cancer cells of magnetite nanoflowers with and without encapsulation in PS-b-PAA block copolymer micelles - Institut de Biologie Paris Seine
Article Dans Une Revue ACS Applied Materials & Interfaces Année : 2024

Biodegradation by cancer cells of magnetite nanoflowers with and without encapsulation in PS-b-PAA block copolymer micelles

Résumé

Magnetomicelles were produced by the self-assembly of magnetite iron oxide nanoflowers and the amphiphilic poly(styrene)-b-poly(acrylic) acid block copolymer to deliver a multifunctional theranostic agent. Their bioprocessing by cancer cells was investigated in a three-dimensional spheroid model over a 13-day period, and compared with non-encapsulated magnetic nanoflowers.

A degradation process was identified and monitored at various scales, exploiting different physicochemical fingerprints. At a collective level, measurements were conducted using magnetic, photothermal, and magnetic resonance imaging techniques. At the nanoscale, transmission electron microscopy was employed to identify the morphological integrity of the structures, while X-ray absorption spectroscopy was used to analyze the degradation at the crystalline phase and chemical levels. All these measurements converge to demonstrate that the encapsulation of magnetic nanoparticles in micelles effectively mitigates their degradation compared to individual non-encapsulated magnetic nanoflowers. This protective effect consequently resulted in better maintenance of their therapeutic photothermal potential. The structural degradation of magnetomicelles occurred through the formation of an oxidized iron phase in ferritin from the magnetic nanoparticles, leaving behind empty spherical polymeric ghost shells. These results underscore the significance of encapsulation of iron oxides in micelles in preserving nanomaterial integrity and regulating degradation, even under the challenging physicochemical conditions within cancer cells.

Fichier principal
Vignette du fichier
Benassai et al final ACS AMI_revised_non highlighted copie.pdf (5.17 Mo) Télécharger le fichier
Origine Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-04696601 , version 1 (13-09-2024)

Identifiants

  • HAL Id : hal-04696601 , version 1

Citer

Emilia Benassai, Niéli Daffé, Elif Aygun, Audrey Geeverding, Emine Ulku, et al.. Biodegradation by cancer cells of magnetite nanoflowers with and without encapsulation in PS-b-PAA block copolymer micelles. ACS Applied Materials & Interfaces, 2024. ⟨hal-04696601⟩
0 Consultations
0 Téléchargements

Partager

More